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Created by J yadonknow
almost 8 years ago
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| Question | Answer |
| Acetobacter | Acetic acid Obligate aerobes |
| Vitamin C | Acetobacter incomplete [O] |
| AB | Streptomyces |
| Swiss cheese | propionic acid bacteria |
| Insulin efficiency | Small peptide Easier to construst aGene than making from larger precursor protein |
| Codon bias | Codon usage preference varies btw. org Many need to alter codons used to fit w/ those recognised by your bacterial species |
| Other problems w/ mammalian genes in bacteria | Protein may require post-translational mod May be degraded by host May be toxic to host |
| Production of metabolites requires | Single gene won't suffice Build up whole metabolic pathway |
| Pathway engineering | Assemble met. pathway using genes from 1 or more org. to produce large amounts of metabolite |
| Indigo production (4) | tryptophan tryptophanase in E.coli Indole Napthalene oxygenase from pseudomonas |
| Biomediation | Org. to clean up enviro pollutants |
| Microbial plastic | PHAs |
| Microbial leaching | Low grade ore crushed + dumped in pattern w/ ^ SA Fe3+ crucial [RA] |
| Metagenomes | Collective genomes of all organisms growing in an environment |
| Gene mining | Isolate potentially useful novel genes w/o having to culture the org. |
| Targeted gene mining | Metagenomics used to screen directly for Ez. w/ certain properties. |
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