s294

Question	Answer
electron microscopy	magnify up to 100000 times. visualise individual features. dead cells. ultra-thin sections. stained with heavy metals that bind to structures, helping discern electron-dense areas of detail. electrons transmitted through and reflected off of specimen.
homogenised	disrupted to form a mixture of subcellular components. cell fragments, proteins and other cell constituents.
cell fractionation	centrifugation to separate individual cell components such as organelles from a cell homogenate according to their respective mass or density.
ion-exchange chromatography	used to purify a particular protein from a mixture on the basis of charge
gel filtration	chromatography tecnique, proteins purified by size

Resource summary

	Created by Tiff Whiff about 6 years ago