19626025
| Question | Answer |
| RFLP | Restriction Fragment Length Polymorphism |
| VNTR | variable number tandem repeat |
| RFLP compared to PCR | not sensitive, 25ng+ DNA needed, sensitive to degradation (alleles are lg), labour intensive & time-consuming |
| PM+DQA1 | Polymarker plus DQA1: limited discrimination 1-2 ng needed smaller frags amplified (242-245 bases) labor intensive |
| AMPFLPs | Amplified Fragment Length Polymorphisms poor discrimination ranged in size 200-1000bp |
| STR | Short Tandem Repeats or microsatellites |
| STR multiplexes | simultaneous analysis high discrimination 0.5-1 ng DNA 100-300 bp range automation |
| CODIS | Combined DNA Index System: DBA database - FBI program 3 tiers: local, state & national (USA) // 2 tiers: local national (CAD) as of Jan 2017 - 20 CORE loci (orig. 13 loci) |
| DNA Transfer | not whether DNA is from a person but rather HOW it got to where it was found; DNA can be transferred directly or indirectly |
| Touch DNA | DNA from epithelial cells, small amounts of DNA, Factors: Shredders vs. Non-Shedders, type of surface, length of time, previous handlers, presence of other body fluids |
| Locard's Exchange Principle | contact btw 2 items there will ALWAYS be an exchange of material |
| Standards for Comparison | 1. cannot enter vic profile into CODIS 2. use elimination samples to prevent non-suspect from being entered 3. direct comparison btw suspect and unknown when eliminations not possible 4. (CAD) request removal of profiles uploaded in error |
| Primary Transfer | transfer of DNA from person to object |
| Secondary Transfer | DNA is on 1 item/person and is transferred to a different item/person (ex. Person A, sweaty, shakes hands w\ person B, B opens door handle - person A’s DNA is found on door handle) |
| Sample Collection | cutting, swabbing, dry swab, wet/dry swab |
| SWGDAM | Scientific Working Group on DNA Analysis Method |
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