| Question | Answer | 
| which chemical treatment is used to separate purines from the DNA sequence in Maxam and Gilbert sequencing? | formic acid | 
| which chemical treatment is used in Maxam and Gilbert sequencing to separate guanine from the DNA sequence? | dimethyl sulphate | 
| which chemical treatment is used to separate pyrimidines from the DNA sequence in Maxam and Gilbert sequencing? | hydrazine | 
| what compound is added to hydrazine to prevent the removal of thymine? | NaCl sodium chloride | 
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